Use of the QuikChange Multi SiteDirected Mutagenesis Kit , catalog #200514 and catalog #200515, – by commercial entities requires a commercial license from Agilent Technologies. The QuikChange Multi Site-Directed Mutagenesis Kit , catalog #200513, is presently offered for sale to commercial entities with a limited use license.
Our original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cloning into M13-based bacteriophage vectors and for ss-DNA rescue. This allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA.
2 QuikChange® Multi Site-Directed Mutagenesis Kit INTRODUCTION The QuikChange® Multi site-directed mutagenesis kit * offers a rapid and reliable method for site-directed mutagenesis of plasmid DNA at up to five different sites simultaneously. A single mutagenic oligonucleotide is required to mutagenize each site, using a double-stranded DNA …
The Agilent QuikChange Multi site-directed mutagenesis kits offer the same benefits of simplicity and reliability afforded by the original QuikChange kit , but are based on a novel technology that allows mutagenesis at multiple sites in a single round, using a single oligonucleotide per site. No, b The QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Catalog #210516) contains enough reagents for10 total reactions, which includes 2 control reactions. c Thaw thedNTP mix once, prepare single-use aliquots, and store the aliquots at –20°C.
QuikChange | Agilent, Manual: QuikChange® Site-Directed Mutagenesis Kit, QuikChange Lightning | Agilent, QuikChange | Agilent, Agilent technologies quikchange lightning multi site directed mutagenesis kit Quikchange Lightning Multi Site Directed Mutagenesis Kit , supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 286 PubMed citations. ZERO BIAS – scores, article reviews, protocol conditions and more, a The QuikChange Site-Directed Mutagenesis Kits contain enough reagents for 5 control reactions. b See Preparation of Media and Reagents. c Thaw the dNTP mix once, prepare single-use aliquots, and store the aliquots at –20°C. Do not subject the dNTP mix to multiple freeze-thaw cycles.
difficult targets, Stratagene offers the QuikChange ® XL site directed mutagenesis kit (Catalog #200516). The QuikChange site-directed mutagenesis kit is used to make point mutations, switch amino acids, and delete or insert single or multiple amino acids. The QuikChange site-directed mutagenesis method is performed, The QuikChange Lightning is a site directed mutagenesis (SDM) kit based on a linear amplification method in which mutation-containing primers are incorporated but not copied. The kit performs single and multiple site-directed mutagenesis in less than 3 hours. Find out more here.
Yes, I would recommend using the commercial QuikChange Multi Site-Directed Mutagenesis Kit . They even have an online primer design tool where you can enter your amino acid or nucleotide sequence …
